As a pivotal surface marker present on T cells, The CD3 antigen plays a crucial role in T cell activation, proliferation, and other essential processes, rendering it a highly favored target for bispecific antibodies. GemPharmatech developed the BALB/c-hCD3EDG model carrying human CD3E, CD3D, CD3G and mouse Cd3e, Cd3d, Cd3g removed. The BALB/c-hCD3EDG mice successfully express human CD3E/CD3D/CD3G on the T cell surface and exhibit a normal immune system.
【Case Study: Preclinical in vivo efficacy evaluation of anti-CD3/CD20 BsAb 】
BALB/c-hCD3EDG mice (6-8weeks) were subcutaneously inoculated with murine lymphoma cell line A20-hCD20 cells. Mice were randomly grouped when the average tumor volume reached 90mm3 and divided into a vehicle group and an anti-hCD3/hCD20 (Mosunetuzumab analogue) treated group (n=6). The test article was administered twice every week for a total of 6 times.
Treatment of anti-hCD3/hCD20 showed significant tumor growth inhibition (TGI=81.46%, left). Mice from both groups showed overall good health as shown by their body weight (right). The results demonstrated that BALB/c-hCD3EDG mice are an excellent model for assessing the in vivo efficacy of human CD3-bispecific antibodies.
【Resources of TAA-humanized mice for toxicity research 】
| Strain Number | Strain Name | Availability | Strain Number | Strain Name | Availability |
| T009819 | BALB/c-hHER2 | IF | T057420 | BALB/c-hCD73/hCD3EDG | IF |
| T036851 | BALB/c-hMSLN | IF | T058753 | B6-hCEACAM5(BAC-TG) | IF |
| T050041 | BALB/c-hCD3/hMSLN | IF | T058817 | BALB/c-hGPRC5D/hCD3EDG | IF |
| T054078 | BALB/c-hBCMA | IF | T058940 | BALBc-hCD3EDG/hCD22 | IF |
| T054196 | BALB/c-hFLT3 | IF | T054279 | BALB/c-hCLL1 | RD |
| T054197 | BALB/c-hCD22 | IF | T056080 | BALB/c-hSSTR2 | RD |
| T054198 | BALB/c-hGPC3 | IF | T057498 | BALB/c-hCD20 | RD |
| T054537 | BALB/c-hCD138 | IF | T057695 | BALB/c-hSIGLEC6 | RD |
| T055360 | BALB/c-hGPRC5D | IF | T057818 | BALB/cJGpt-hDLK1 | RD |
| T056083 | BALB/c-hLRRC15 | IF | T057819 | BALB/c-hCD3/hCD5 | RD |
| T056326 | B6-hDLK1 | IF | T057924 | BALB/c-hCD5 | RD |
| T056521 | B6-hCD5 | IF |
GemPharmatech has an extensive collection of syngeneic cell lines, including murine-derived cells and genetic modification of the corresponding cells.
